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Simple broad‐spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed‐field gel electrophoresis analysis
Author(s) -
Oana Kozue,
Kawakami Yoshiyuki,
Hayashi Tetsuya,
Ohnishi Makoto
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2008.00084.x
Subject(s) - lysis , biology , gel electrophoresis , dna , genomic dna , simple (philosophy) , pulsed field gel electrophoresis , electrophoresis , microbiology and biotechnology , computational biology , chromatography , genetics , chemistry , genotype , gene , philosophy , epistemology
We have developed a simple broad‐spectrum protocol using labiase for bacterial cell lysis in pulsed‐field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram‐negative and ‐positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.