Premium
Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood
Author(s) -
Moriyama Kosei,
Ando Chie,
Tashiro Kosuke,
Kuhara Satoru,
Okamura Seiichi,
Nakano Shuji,
Takagi Yasumitsu,
Miki Takeyoshi,
Nakashima Yoshiyuki,
Hirakawa Hideki
Publication year - 2008
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2008.00048.x
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , serratia , polymerase chain reaction , ribosomal rna , clostridium , bacteroides , gene , bacteria , genetics , pseudomonas
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35–40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides‐clostridium‐lactobacillus cluster, which is indigenous to gastrointestinal flora.