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Rapid culture‐free identification and molecular typing of Shiga toxin‐producing Escherichia coli by PCR‐RFLP
Author(s) -
Shima Kensuke,
Kawamura Naohisa,
Hinenoya Atsushi,
Sugimoto Norihiko,
Wu Yuluo,
Asakura Masahiro,
Nishimura Kazuhiko,
Nair Gopinath B.,
Yamasaki Shinji
Publication year - 2008
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2008.00038.x
Subject(s) - restriction fragment length polymorphism , biology , typing , shiga like toxin , microbiology and biotechnology , feces , escherichia coli , polymerase chain reaction , identification (biology) , shiga toxin , toxin , genetics , gene , botany
Using culture‐independent technology, PCR‐RFLP were used to identify and type STEC in the stool of a patient with HUS. Fecal PCR‐RFLP patterns were identical to those of the STEC O157:H7 isolated from the patient.