Premium
Genetic diversity of Francisella tularensis subspecies holarctica strains isolated in Japan
Author(s) -
Fujita Osamu,
Uda Akihiko,
Hotta Akitoyo,
Okutani Akiko,
Inoue Satoshi,
Tanabayashi Kiyoshi,
Yamada Akio
Publication year - 2008
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2008.00036.x
Subject(s) - multiple loci vntr analysis , biology , francisella tularensis , subspecies , genetic diversity , genetics , typing , leptospira , tularemia , variable number tandem repeat , genotype , microbiology and biotechnology , virology , zoology , virulence , gene , leptospirosis , demography , population , sociology
The recently developed MLVA has high discriminatory power for the typing of individual strains or isolates of Francisella tularensis . In the present study, MLVA was applied to 33 Japanese F. tularensis subspecies holarctica strains to examine the genetic diversity of F. tularensis isolated. Among the seven VNTR loci analyzed, Ft‐M2, Ft‐M10, and Ft‐M20 loci showed high genetic polymorphism in Japanese strains, whereas Ft‐M3 was most variable in non‐Japanese strains. These results provide novel extended information about the genomic diversity among the strains of F. tularensis ssp. holarctica distributed in Japan and enable determination of whether a given isolate is indigenous to Japan by examining these loci using MLVA.