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Distinct immunohistochemical localization in Kuru plaques using novel anti‐prion protein antibodies
Author(s) -
Hosokawa Tomoko,
Ono Fumiko,
Tsuchiya Kotaro,
Sato Ichiro,
Takeyama Natsumi,
Ueda Susumu,
Zanusso Gianluigi,
Takahashi Hidehiro,
Sata Tetsutaro,
Sakudo Akikazu,
Suguira Katsuaki,
Baj Andreina,
Toniolo Antonio,
Yoshikawa Yasuhiro,
Onodera Takashi
Publication year - 2008
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2008.00007.x
Subject(s) - scrapie , epitope , monoclonal antibody , biology , immunohistochemistry , antibody , virology , kuru , microbiology and biotechnology , prnp , prion protein , pathology , immunology , biochemistry , medicine , allele , disease , gene
By immunizing Prnp ‐knockout mice with synthetic polypeptides, a panel of mAbs directed to bovine PrP C was obtained. The mAb panel was characterized by the ELISA method, where synthetic polypeptides were used for epitope mapping. Different reactivity patterns were identified. The ability of these mAbs to detect abnormal PrP Sc in CJD cases was studied by immunohistochemistry. All mAbs were tested for PrP Sc in murine, bovine, monkey and human brain tissues. Three mAbs recognized the fragmented PrP epitope in our ELISA. Antibody 1D12 was strongly reactive to ovine and squirrel monkey tissues infected with a scrapie agent, although non‐reactive to scrapie‐infected mouse tissues. Antibody 2D8 was clearly reactive to type‐2 but not type‐1 CJD human tissues. Of particular interest was the reactivity of mAb 6C4 with the inner structure of Kuru plaques (peripheral pattern) in a type‐2 CJD case and mAb T2, 1D12, 2B11, 2D8, 4B5 and 6G3‐2 with the central area (central pattern). The fact that different anti‐PrP mAbs possess distinct staining properties suggests that the PrP c to PrP Sc conversion might involve a multiple‐step process.