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First Molecular Detection of Borrelia afzelii in Clinical Samples in Korea
Author(s) -
Choi YeonJoo,
Han SeungHoon,
Park JinMi,
Lee KyungMin,
Lee EunMi,
Lee SeungHyun,
Song HyeonJe,
Koh YoungSang,
Lee KeunWha,
Jang WonJong,
Park KyungHee
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb04015.x
Subject(s) - biology , borrelia afzelii , borrelia burgdorferi , lyme disease , restriction fragment length polymorphism , virology , borrelia garinii , borrelia , antibody , antigen , dna , nucleic acid , sensu , polymerase chain reaction , microbiology and biotechnology , genetics , gene , botany , genus
Borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested PCR that targeted the rrf (5S)‐ rrl (23S) spacer of B. burgdorferi sensu lato. The B. afzelii ‐specific DNA fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin G or M antibodies against B. burgdorferi antigens through IFA. The results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the DNA fragments. The results indicated for the first time that Lyme borreliosis is prevalent in Korea.