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Exposure to Leishmania major Modulates the Proportion of CD4T Cells without Affecting Cellular Immune Responses
Author(s) -
Alimohammadian Mohammad Hossein,
Darabi Haiedeh,
Malekzadeh Shirin,
MahmoodzadehNiknam Hamid,
Ajdary Soheila,
Khamesipour Ali,
Bahonar Ahmad,
Mofarrah Alireza
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03984.x
Subject(s) - biology , immune system , leishmania , immunology , virology , parasite hosting , computer science , world wide web
The immune responses of individuals exposed to Leishmania major were evaluated and compared with those of non‐exposed volunteers. Forty‐one patients with active lesion(s), 43 healed individuals, 15 vaccinees 1 month or 1 year post vaccination, and 15 non‐exposed volunteers were studied. Leishmanin skin test (LST) response, proliferative response of lymphocyte (PRL) to L. major antigen, IFN‐γ and IL‐4 production, and percentage of L. major ‐specific CD4 + , CD8 + and CD16/CD56 + cells in peripheral blood mononuclear cells were assessed. Data showed positive LST (>5 mm) in 92% of patients, 98% of healed, and 80% or 43% of vaccinees 1 month and 1 year post vaccination, respectively. Positive PRL (SI>2.5) was displayed in 90%, 84%, 46% and 7% of patients, healed, vaccinated (post 1 year) and non‐exposed donors, respectively. The mean ±S.E. of IFN‐γ was 924 ± 149, 1,278 ± 185, 470 ± 282 or 258 ± 82 pg/ml in patients, healed cases and vaccinees after 1 month or 1 year, respectively. Positive IFN‐γ responders (>300 pg/ml) were shown in 72% of patients, 81% of healed cases, 31% or 39% of vaccinees and 0% of non‐exposed donors. A reduced percentage of CD4 + T‐cells and an increased percentage of NK cells were found in exposed individuals compared to non‐exposed donors. The data indicated that exposure to L. major modulates the proportion of CD4 + T cells and increases NK cells percentage. However, the cellular immune responses including induction of LST, and IFN‐γ production are increased in exposed individuals.