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In Vitro Susceptibilities of Candida spp. to Panomycocin, a Novel Exo‐β‐1,3‐Glucanase Isolated from Pichia anomala NCYC 434
Author(s) -
İzgü Fatih,
Altınbay Demet,
Türeli Akif Emre
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03975.x
Subject(s) - candida krusei , biology , microbiology and biotechnology , anomala , candida glabrata , candida parapsilosis , candida albicans , pichia , candida tropicalis , broth microdilution , corpus albicans , fungi imperfecti , minimum inhibitory concentration , antibiotics , pichia pastoris , biochemistry , botany , recombinant dna , gene
Panomycocin, the killer toxin of Pichia anomala NCYC 434 (K5), is a 49 kDa monomeric glycoprotein with exo‐β–1,3‐glucanase activity (patent pending). In this study we evaluated the in vitro activity of panomycocin against a panel of 109 human isolates of seven different pathogenic Candida spp. using microdilution and time‐kill methods. Panomycocin was most active against C. tropicalis, C. pseudotropicalis and C. glabrata with MIC 90 values of 1 μg/ml. It displayed significant activity against C. albicans and C parapsilosis with MIC 90 values of 4 and 2 μg/ml, respectively. For C. krusei , the MIC 90 value was 8 μg/ml. Panomycocin was fungicidal against all the tested Candida spp. The MFC values were only one or 2 dilutions higher than the MICs with the exception of C. krusei isolates with MFCs greater than or equal to 4 × MIC. Results of this study indicated that panomycocin could be considered as a natural antifungal agent against Candida infections and has significant potential for further investigation.