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Development of an Immunochromatographic Kit for Rapid Diagnosis of H5 Avian Influenza Virus Infection
Author(s) -
Tsuda Yoshimi,
Sakoda Yoshihiro,
Sakabe Saori,
Mochizuki Tsuyoshi,
Namba Yasuharu,
Kida Hiroshi
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03973.x
Subject(s) - influenza a virus subtype h5n1 , virology , virus , biology , monoclonal antibody , immunoassay , highly pathogenic , antibody , avian influenza virus , antigen , influenza a virus , h5n1 genetic structure , microbiology and biotechnology , immunology , medicine , covid-19 , pathology , disease , infectious disease (medical specialty)
Highly pathogenic avian influenza (HPAI) caused by the H5N1 subtype has given rise to serious damage in poultry industries in Asia. The virus has expanded its geographical range to Europe and Africa, posing a great risk to human health as well. For the control of avian influenza, a rapid diagnosis by detecting the causative virus and identifying its subtype is essential. In the present study, a rapid diagnosis kit combining immunochromatography with enzyme immunoassay which detects the H5 HA antigen of influenza A virus was developed using newly established anti‐H5 HA monoclonal antibodies. The present kit specifically detected all of the H5 influenza viruses tested, and did not react with the other HA subtypes. H5 HA antigens were detected from swabs and tissue homogenates of chickens infected with HPAI virus strain A/chicken/Yamaguchi/7/04 (H5N1) from 2 days post inoculation. The kit showed enough sensitivity and specificity for the rapid diagnosis of HPAI.