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Identification of Enterohemorrhagic Escherichia coli O157‐Specific DNA Sequence Obtained from Amplified Fragment Length Polymorphism Analysis
Author(s) -
Tokunaga Akihiko,
Kawano Masanori,
Okura Masatoshi,
Iyoda Sunao,
Watanabe Haruo,
Osawa Ro
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03970.x
Subject(s) - biology , escherichia coli , genetics , locus (genetics) , restriction fragment length polymorphism , microbiology and biotechnology , gene , sequence analysis , dna , fragment (logic) , polymorphism (computer science) , genetic marker , cleaved amplified polymorphic sequence , polymerase chain reaction , genotype , computer science , programming language
An approximately 1.1 kbp fragment that was commonly observed only in the enterohemorrhagic Escherichia coli (EHEC) O157 strains in an analysis of amplified fragment length polymorphism was found to be a partial gene sequence encoding the locus of toxB and a useful molecular marker for the identification of EHEC O157.