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Evaluation of Repetitive Extragenic Palindromic‐PCR for Discrimination of Fecal Escherichia coli from Humans, and Different Domestic‐ and Wild‐Animals
Author(s) -
Mohapatra Bidyut R.,
Broersma Klaas,
Nordin Rick,
Mazumder Asit
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03962.x
Subject(s) - biology , feces , polymerase chain reaction , escherichia coli , dna profiling , primer (cosmetics) , veterinary medicine , microbiology and biotechnology , zoology , genetics , dna , gene , medicine , chemistry , organic chemistry
The objective of this study was to investigate the potential of repetitive extragenic palindromic anchored polymerase chain reaction (rep‐PCR) in differentiating fecal Escherichia coli isolates of human, domestic‐ and wild‐animal origin that might be used as a molecular tool to identify the possible source(s) of fecal pollution of source water. A total of 625 fecal E. coli isolates of human, 3 domestic‐ (cow, dog and horse) and 7 wild‐animal (black bear, coyote, elk, marmot, mule deer, raccoon and wolf) species were characterized by rep‐PCR DNA fingerprinting technique coupled with BOX A1R primer and discriminant analysis. Discriminant analysis of rep‐PCR DNA fingerprints of fecal E. coli isolates from 11 host sources revealed an average rate of correct classification of 79.89%, and 84.6%, 83.8%, 83.3%, 82.5%, 81.6%, 80.8%, 79.8%, 79.3%, 77.4%, 73.2% and 63.6% of elk, human, marmot, mule deer, cow, coyote, raccoon, horse, dog, wolf and black bear fecal E. coli isolates were assigned to the correct host source. These results suggest that rep‐PCR DNA fingerprinting procedures can be used as a source tracking tool for detection of human‐ as well as animal‐derived fecal contamination of water.