Premium
In Vitro Th1 Cytokine‐Independent Th2 Suppressive Effects of Bifidobacteria
Author(s) -
Iwabuchi Noriyuki,
Takahashi Noritoshi,
Xiao Jinzhong,
Miyaji Kazuhiro,
Iwatsuki Keiji
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03953.x
Subject(s) - biology , immune system , bifidobacterium longum , cytokine , bifidobacterium , ovalbumin , antigen , immunology , in vitro , interleukin , microbiology and biotechnology , bacteria , lactobacillus , biochemistry , genetics
A comparison between 17 strains of lactic acid bacteria and 15 strains of bifidobacteria indicated that bifidobacteria induced significantly lower levels of interleukin‐12 (IL‐12) in murine splenic cells. The present study aims to evaluate the effect and mechanism of Bifidobacterium longum BB536, a probiotic strain, in suppressing antigen‐induced Th2 immune response in vitro . BB536 suppressed immunoglobulin (Ig) E and IL‐4 production by ovalbumin‐sensitized splenic cells, but induction of Th1‐inducing cytokine production, such as IL‐12 and gamma interferon (IFN‐γ) tended to be lower compared with lactic acid bacteria. Neutralization with antibodies to IL‐12, IFN‐γ, IL‐10 and transforming growth factor β indicated negative involvement of Th1‐inducing cytokines and regulatory cytokines in the suppression of Th2 immune response by BB536, especially when treated at higher doses of BB536 (>10 μg cells/ml). Furthermore, BB536 induced the maturation of immature bone marrow‐derived dendritic cells (BM‐DCs), and suppressed antigen‐induced IL‐4 production mediated by BM‐DCs. These results suggested that BB536 suppressed Th2 immune responses, partially independent of Th1‐inducing cytokines and independent of regulatory cytokines, mediated by antigen‐presenting cells such as dendritic cells.