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Simultaneous Detection and Genogroup‐Screening Test for Norovirus Genogroups I and II from Fecal Specimens in Single Tube by Reverse Transcription‐Loop‐Mediated Isothermal Amplification Assay
Author(s) -
Fukuda Shinji,
Sasaki Yukie,
Kuwayama Masaru,
Miyazaki Kazuo
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03932.x
Subject(s) - reverse transcription loop mediated isothermal amplification , loop mediated isothermal amplification , norovirus , biology , feces , virology , agarose , microbiology and biotechnology , reverse transcriptase , gene , virus , dna , genetics , polymerase chain reaction
We developed a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup‐specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT‐LAMP assay for the simultaneous detection of NoV genogroup I (GI) and II (GII) genomes in a single tube. The genogrouping was achieved by using fluorescence‐labeled primers, and the green and red colors for GI and GII, respectively, sometimes with yellow color for GI and GII mixture, were indicated on the agarose gel under UV light at 312 nm.