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Isolation and Characterization of Virulent Yellowtail Ascites Virus
Author(s) -
Hirayama Takeshi,
Nagano Ichiro,
Shinmoto Hajime,
Yagyu Kenichi,
Oshima Syunichirou
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03927.x
Subject(s) - biology , virulence , seriola quinqueradiata , ascites , virus , virology , tilapia , microbiology and biotechnology , fish <actinopterygii> , fishery , gene , genetics , medicine
Yellowtail ascites virus (YTAV) is the causative agent of ascites and deformity in fish and causes serious losses to the fish‐farming industry of yellowtail fry and fingerling Seriola quinqueradiata in Japan. In 2006, cultured yellowtail died from ascites in Kochi, Japan. We isolated and characterized a virus from the diseased fish. Based on the pathogenicity, culture characteristics, morphological features, RT‐PCR results targeting VP2/NS region, phylogeny based on the VP1 amino acid sequence, and immunochemical reactivity of structural proteins, the virus isolate was identified as YTAV (designated as YTAV‐06). YTAV‐06 was a more virulent isolate than YTAV Y‐6, isolated originally from yellowtail with ascites. To our knowledge, this is the first report describing that YTAV isolates may vary in their virulence.