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Interferon Gamma Response to Combinations 38 kDa/CFP‐10, 38 kDa/MPT‐64, ESAT‐6/MPT‐64 and ESAT‐6/CFP‐10, Each Related to a Single Recombinant Protein of Mycobacterium tuberculosis in Individuals from Tuberculosis Endemic Areas
Author(s) -
Tavares Ricardo C. Oliveira,
Salgado Jorge,
Moreira Valéria Barbosa,
Ferreira Mônica Antonia S.,
Mello Fernanda C. Queiroz,
Leung Janaína W.,
Fonseca Leila de Souza,
Spallek Ralf,
Singh Mahaviir,
Saad Maria Helena F.
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03910.x
Subject(s) - esat 6 , antigen , mycobacterium tuberculosis , tuberculin , tuberculosis , immunology , immune system , biology , interferon gamma , microbiology and biotechnology , medicine , pathology
Several antigens of Mycobacterium tuberculosis have been identified and specificity to one or multiple antigens could determine the distinction between protective and pathogenic host reaction. Therefore T cell immune response to combinations 38 kDa/CFP‐10, 38 kDa/MPT‐64, ESAT‐6/MPT‐64 and ESAT‐6/CFP‐10 (each related to a single protein of Mycobacterium tuberculosis ) in individuals from tuberculosis endemic areas have been examined. ELISA was used to detect IFN‐γ production in PBMC priming with single proteins and combinations in a panel of 105 individuals: 38 tuberculosis patients (6 untreated and 32 treated) and 67 healthy controls with tuberculin skin test positive or negative (TST). Brazilian TB patients highly recognized ESAT‐6 (66%), but combinations improved response in the following order: ESAT‐6/MPT‐64 (89%) > ESAT‐6/CFP‐10 (73%) > 38 kDa/CFP‐10 (70%), the last combination showing the highest specificity (TST + =42% and TST – =83%). Average IFN‐γ production in TB patients was significantly higher for 38 kDa/CFP‐10 ( P =0.012) and 38 kDa/MPT‐64 ( P <0.035), when compared to single antigens. None of the combinations was able to discriminate TB patients from TST + controls; however, 38 kDa/CFP‐10 displayed a borderline significance ( P =0.053). Similar to the ESAT‐6/CFP‐10 combination, IFN‐γ response to 38 kDa/CFP‐10 showed an increased tendency in treated patients, although not significant ( P =0.16). We demonstrated for the first time that 38 kDa/CFP‐10 had prediction sensitivity for TB patients similar to the ESAT‐6/CFP‐10 combination and also significant response improvement related to the single proteins with more selective reactivity among TST‐positive individuals, which could be of potential interest for diagnostic evaluation for tuberculosis infection.