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Molecular Characterization of Imipenem‐Resistant Pseudomonas aeruginosa in Hiroshima, Japan
Author(s) -
Ohara Masaru,
Kouda Shuntaro,
Onodera Makoto,
Fujiue Yoshihiro,
Sasaki Megumi,
Kohara Tadahiro,
Kashiyama Seiya,
Hayashida Shizue,
Kadono Manami,
Komatsuzawa Hitoshi,
Gotoh Naomasa,
Usui Tuguru,
Itaha Hideyuki,
Kuwabara Masao,
Yokoyama Takashi,
Sugai Motoyuki
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03908.x
Subject(s) - imipenem , pseudomonas aeruginosa , microbiology and biotechnology , biology , polymerase chain reaction , levofloxacin , pseudomonadaceae , efflux , drug resistance , antibiotics , gene , bacteria , antibiotic resistance , genetics
Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a–f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla IM P‐1 or bla VIM‐2 allele positive by polymerase chain reaction (PCR). Among 100 imipenem‐resistant strains, 32 were categorized into multi‐drug resistant strains, in which 13 were positive for the metallo‐β‐lactamase gene. Fifty‐one strains (51%) among the 100 imipenem‐resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi‐drug resistant strains. The pulsed field gel electrophoresis analysis of the Spe I‐digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo‐β‐lactamase producing P. aeruginosa strains in Japanese hospitals.