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A New Screening Method to Identify Inhibitors of the Lol (Localization of lipoproteins) System, a Novel Antibacterial Target
Author(s) -
Ito Hideaki,
Ura Atsushi,
Oyamada Yoshihiro,
Yoshida Hiroaki,
Yamagishi Junichi,
Narita Shinichiro,
Matsuyama Shinichi,
Tokuda Hajime
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03906.x
Subject(s) - high throughput screening , escherichia coli , bacteria , biology , gram negative bacteria , antibacterial activity , biochemistry , computational biology , microbiology and biotechnology , gene , genetics
As the Lol system, which is involved in localization of lipoproteins, is essential for Escherichia coli growth and widely conserved among gram‐negative bacteria, it is considered to be a promising target for the development of anti‐gram‐negative bacterial agents. However, no high‐throughput screening method has so far been developed to screen for Lol system inhibitors. By combining three assay systems (anucleate cell blue assay, Lpp assay, and LolA‐dependent release inhibition assay) and a drug susceptibility test, we have successfully developed a new screening method for identification of compounds that inhibit the Lol system. Using this new screening method, we screened 23,600 in‐house chemical compounds and found 2 Lol system inhibitors. We therefore conclude that our new screening method can efficiently identify new antibacterial agents that target the Lol system.