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Effect of Interleukin‐10 on the Paracoccidioides brasiliensis Killing by Gamma‐Interferon Activated Human Neutrophils
Author(s) -
Costa Diego Luís,
DiasMelicio Luciane Alarcão,
Acorci Michele Janegitz,
Bordon Ana Paula,
Tavian Elisandra Garcia,
Peraçoli Maria Terezinha Serrão,
Soares Angela Maria Victoriano
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03892.x
Subject(s) - paracoccidioides brasiliensis , paracoccidioidomycosis , biology , microbiology and biotechnology , paracoccidioides , interferon gamma , phagocytosis , cytokine , macrophage , immunology , interleukin 8 , in vitro , biochemistry
Paracoccidioidomycosis, a deep mycosis endemic in Latin America, is a chronic granulomatous disease caused by the fungus Paracoccidioides brasiliensis . Phagocytic cells play a critical role against this fungus, and several studies have shown the effects of activator and suppressive cytokines on macrophage and monocyte functions. However, studies on polymorphonuclear neutrophils (PMNs), that are the first cells recruited to the infection sites, are scarcer. Thus, the objective of this paper was to assess whether interleukin‐10 (IL‐10), a potent anti‐inflammatory cytokine, is able to block the activity of IFN‐γ‐activated human PMNs upon P. brasiliensis intracellular killing, in vitro . The results showed that IFN‐γ‐activated PMNs have an effective fungicidal activity against the fungus. This activity was associated with the release of high levels of H 2 O 2 , the metabolite involved in phagocytic cells antifungal activities. However, the concomitant incubation of these cells with IFN‐γ and IL‐10 significantly blocked IFN‐γ activation. As a consequence, PMNs killing activity and H 2 O 2 release were inhibited. Together, our results show the importance of PMNs exposure to activator or suppressor cytokines in the early stages of paracoccidioidomycosis infection.