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Direct Application of Ava II PCR Restriction Fragment Length Polymorphism Analysis ( Ava II PRA) Targeting 644 bp Heat Shock Protein 65 ( hsp65 ) Gene to Sputum Samples
Author(s) -
Mun HoSuk,
Kim HyunJu,
Oh EunJu,
Kim Hong,
Park YoungGil,
Bai GilHan,
Do Junghwan,
Cha ChangYong,
Kook YoonHoh,
Kim BumJoon
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03880.x
Subject(s) - sputum , restriction fragment length polymorphism , biology , microbiology and biotechnology , polymorphism (computer science) , polymerase chain reaction , gene , genotype , pathology , genetics , tuberculosis , medicine
To evaluate the usefulness of the AvaII PRA method targeting 644‐bp hsp65 gene for the direct detection of pathogenic mycobacteria from clinical specimens, we applied this method to 40 sputum samples and compared the results to those obtained by IS6110 PCR. Although this method showed a sensitivity slightly lower than IS6110 PCR (97.5% vs. 100%), it detected infections of M. avium complex (MAC) in two patients, which was not possible by IS6110 PCR. We conclude that AvaII PRA is a highly effective method for directly detecting pathogenic mycobacteria in primary clinical specimens.