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Prolonged Viral RNA Detection in Blood and Lymphoid Tissues from Coxsackievirus B4 E2 Orally‐Inoculated Swiss Mice
Author(s) -
Jaïdane Hela,
Gharbi Jawhar,
Lobert PierreEmmanuel,
Lucas Bernadette,
Hiar Raïda,
M'Hadheb Manel Ben,
Brilot Fabienne,
Geenen Vincent,
Aouni Mahjoub,
Hober Didier
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03874.x
Subject(s) - coxsackievirus , biology , spleen , rna , virology , immunology , virus , enterovirus , gene , biochemistry
The spreading of viral RNA within Swiss Albino mice orally inoculated with coxsackievirus B4 E2 strain (CVB4 E2) was studied by using RT‐PCR and semi‐nested‐RT‐PCR methods. Viral RNA was detected in various organs: pancreas, heart, small intestine, spleen, thymus, and blood at various postinfectious (p.i.) times ranging from 8 hr to 150 days. Our results show that (i) outbred mice can be infected with CVB4 E2 following an oral inoculation, which results in systemic spreading of viral RNA, (ii) CVB4 E2 infection can be associated with a prolonged detection of viral RNA in spleen, thymus and blood, up to 70 days p.i. and further in other organ tissues.