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Proteolytic Activation of Vibrio mimicus (Vm) Major Outer Membrane Protein Haemagglutinin (HA) with Vm‐HA/Protease: Implication for Understanding Bacterial Adherence
Author(s) -
Alam Munirul,
Miyoshi Shinichi,
Ahmed Kabir Uddin,
Hasan Nur A.,
Tomochika Kenichi,
Shinoda Sumio
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03859.x
Subject(s) - protease , proteases , proteolysis , biology , microbiology and biotechnology , bacterial outer membrane , bacteria , vibrio cholerae , biochemistry , enzyme , escherichia coli , genetics , gene
Vibrio mimicus (Vm) haemagglutinins (HAs), such as an extracellular HA/protease (Vm‐HA/protease) and a major outer membrane protein‐HA (Vm‐OMPHA), have been recognized as the putative adherence factors for the bacterium. However, the mechanism by which HAs coordinate the adherence function of the bacterium remains as yet unknown. We report herein the positive interaction between Vm‐HA/protease and Vm‐OMPHA resulting in significant enhancement of the haemagglutinating ability. In this interaction, no cleaved polypeptide was detected; however, limited proteolysis of Vm‐OMPHA was confirmed by SDS‐PAGE. The proteolytic activation of the native cell‐associated Vm‐OMPHA by limited proteolysis was also demonstrated in several V. mimicus strains. Proteolytic activation of OMPHA was also achieved with various proteases from bacterial and eukaryotic sources. These findings may indicate a novel coordination of V. mimicus HAs in the adherence of the bacterium.