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Evidence for Pestivirus Infection in Free‐Living Japanese Serows, Capricornis crispus
Author(s) -
Harasawa Ryô,
Aoyama Fumio,
Goryo Masanobu,
Okada Kosuke,
Nishimura Takashi,
Matsubara Kazuei,
Tsujimoto Tsunenori,
Giangaspero Massimo
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03858.x
Subject(s) - biology , pestivirus , virology , virus , flaviviridae , nucleotide , nucleic acid sequence , genetics , viral disease , gene
Sixteen serum samples collected from free‐living Japanese serows, Capricornis crispus , between 2001 and 2004 in Morioka and its vicinity were examined for the presence of pestivirus by reverse transcription‐nested PCR procedure. Three out of the 16 samples produced a visible band in electrophoresed agarose gels. The nucleotide sequences of the three PCR products were found to be identical. The pestivirus found in the serow was identified as Bovine viral diarrhea virus 1 (BVDV‐1) based on nucleotide sequence analyses by phylogeny as well as palindromic nucleotide substitutions at the 5′ untranslated regions. Our data first indicated that BVDV‐1 infection occurred continuously among the free‐living serow populations though the role of BVDV‐1 in wild ungulates is currently unknown.