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Human Herpesvirus‐6‐Specific Interleukin 10‐Producing CD4 + T Cells Suppress the CD4 + T‐Cell Response in Infected Individuals
Author(s) -
Wang Fang,
Yao Kun,
Yin QuanZhang,
Zhou Feng,
Ding ChuanLin,
Peng GuangYong,
Xu Jian,
Chen Yun,
Feng DongJu,
Ma ChunLin,
Xu WenRong
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03855.x
Subject(s) - biology , cd8 , il 2 receptor , interleukin 21 , interferon gamma , interleukin 2 , cytotoxic t cell , immunology , microbiology and biotechnology , t lymphocyte , cytokine , t cell , immune system , virology , in vitro , biochemistry
Human herpesvirus‐6 (HHV‐6) infection normally persists for the lifetime of the host and may reactivate with immunosuppression. The mechanism behind HHV‐6 latent infection is still not fully understood. In this study, we observed that decreased proliferation of CD4 + T cells and PBMCs but not CD8 + T cells from HHV‐6‐infected individuals was stimulated with HHV‐6‐infected cell lysates. Moreover, HHV‐6‐stimulated CD4 + T cells from HHV‐6‐infected individuals have suppressive activity on naïve CD4 + T and CD8 + T cells from HHV‐6‐uninfected individuals. However, no increased proportion of CD4 + CD25 + Treg cells from HHV‐6‐infected individuals contributed to the suppressive activity of the HHV‐6‐stimulated CD4 + T cells from HHV‐6‐infected individuals. Transwell experiments, ELISA and anti‐IL‐10 antibody blocking experiment demonstrated that IL‐10 may be the suppressive cytokine required for suppressive activity of CD4 + T cells from HHV‐6‐infected individuals. Results of intracellular interleukin (IL)‐10 and IL‐4 further implicated the HHV‐6‐speciflc IL‐10‐producing CD4 + T cells in the suppressive activity of CD4 + T cells from HHV‐6‐infected individuals. Results of intracellular interferon (IFN)‐γ demonstrated a decreased frequency of HHV‐6‐speciflc IFN‐γ‐producing CD4 + T, but not CD8 + T cells in HHV‐6‐infected individuals, indicating that it was the CD4 + Th1 responses in HHV‐6‐infected individuals that were selectively impaired. Our findings indicated that HHV‐6‐specific IL‐10‐producing CD4 + T cells from HHV‐6‐infected individuals possess T regulatory type 1 cell activity: immunosuppression, high levels of IL‐10 production, with a few cells expressing IFN‐γ, but none expressing IL‐4. These cells may play an important role in latent HHV‐6 infection.