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Genetic Variability of Human Cytomegalovirus UL132 Gene in Strains from Infected Infants
Author(s) -
Sun Zheng Rong,
Ji Yao Hua,
Ruan Qiang,
He Rong,
Ma Yan Ping,
Qi Ying,
Mao Zhi Qin,
Huang Yu Jing,
Wang Yue Ping
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03853.x
Subject(s) - biology , human cytomegalovirus , open reading frame , genetics , gene , phylogenetic tree , genotype , tropism , genetic variation , virology , cytomegalovirus , mega , genetic variability , nucleic acid sequence , herpesviridae , virus , peptide sequence , viral disease , physics , astronomy
Human cytomegalovirus (HCMV) displays genetic polymorphisms. HCMV infects a number of organs and cell types, leading to the hypothesis that HCMV disease and tissue tropism may be related to specific sequence variability. A gene in UL/b′ of HCMV, UL132 open reading frame (ORF), encodes glycoprotein (gpUL132) which is identified as a low‐abundance structural component of HCMV. In this study, the sequence variability of the UL132 gene was studied in 30 clinical strains. The results showed that a large number of nucleotide non‐synonymous substitutions occurred in the UL132 ORF, particularly in the 5′ half, in comparison to the UL132 of reference strain, Toledo. The UL132 variants of the clinical strains were clustered clearly into three major groups in the phylogenetic tree: G1(10/30), G2(9/30), and G3(11/30). The precise definition of UL132 genotypes and their putative functions would be helpful in a better understanding of the HCMV.