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Clostridium glycyrrhizinilyticum Sp. Nov., a Glycyrrhizin‐Hydrolysing Bacterium Isolated from Human Faeces
Author(s) -
Sakuma Keita,
Kitahara Maki,
Kibe Ryoko,
Sakamoto Mitsuo,
Benno Yoshimi
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03818.x
Subject(s) - biology , microbiology and biotechnology , clostridium , 16s ribosomal rna , bacteria , ruminococcus , obligate anaerobe , ribosomal rna , eubacterium , strain (injury) , clostridiaceae , gene , feces , genetics , anatomy
Screening of faecal bacteria for glycyrrhetic acid (GA) production by hydrolysing of glycyrrhizin (GL) resulted in the isolation of two strains, designated ZM35 T and ZM38. Strains ZM35 T and ZM38 were Gram‐positive, obligate anaerobic, non‐spore‐forming and rod‐shaped bacteria. Analysis of the 16S rRNA gene sequences indicated that strains ZM35 T and ZM38 belonged to cluster XIVa of the genus Clostridium . The 16S rRNA gene sequences of strains ZM35 T and ZM38 were identical. Strain ZM35 T exhibited approximately 94% to 95% identity with the validly described species, Clostridium oroticum (94.5%), Eubacterium contortum (93.8%), Ruminococcus gnavus (94.5%) and R. torques (95.1%). In an experiment of DNA‐DNA hybridization, it was confirmed that strains ZM35 T and ZM38 were the same species. The guanine‐plus‐cytosine (G+C) content of strain ZM35 T is 45.7 mol%. Based on the phylogenetic and phenotypic findings, we propose that strains ZM35 T and ZM38 be assigned to a novel species named Clostridium glycyrrhizinilyticum . The type strain is ZM35 T (=JCM 13368 T =DSM 17593 T ).