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Diagnostic Surface Expression of SWAP‐70 on HIV‐1 Infected T Cells
Author(s) -
Kimbara Noriaki,
Dohi Natsuki,
Miyamoto Mizuyu,
Asai Suzuka,
Okada Hidechika,
Okada Noriko
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03790.x
Subject(s) - biology , microbiology and biotechnology , cell culture , cd14 , virology , antibody , complementary dna , flow cytometry , immunology , gene , genetics
Following immunization with HIV‐1 infected cells, a hybridoma cell line termed 9F11 was established from the P3U‐1 myeloma line fused with lymphocytes from a trans‐chromosome (TC) mouse, that harbors human chromosomes containing immunoglobulin genes. The 9F11 human IgM monoclonal antibody (9F11 Ab) reacts with HIV‐1 infected MOLT4 cells but not with uninfected MOLT4 cells, and causes immune cytolysis with homologous human complement at a concentration as low as 0.4 μg/ml. This Ab was used to perform immunoscreening of a cDNA expression library derived from HIV‐1 infected cells. All positive cDNA clones contained SWAP‐70 cDNA. SWAP‐70 RNA and protein expression are much stronger in HIV‐1 infected cells. SWAP‐70 was also detected on the surface of HIV‐1 infected cells by flow cytometric analysis. The monocyte cell line U937 cells expresses SWAP‐70 on its cell surface regardless of whether it was infected with HIV‐1. Furthermore, among PBMCs surface expression of SWAP‐70 was detected on CD21+, CD56+ and CD14+ cells. Although CD3+ cells scarcely express SWAP‐70 on their surface, once activated, they become positive. SWAP‐70 may therefore serve as a marker for T cell differentiation as well as for HIV‐1 infection.