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Nucleolar Protein B23 Interacts with Japanese Encephalitis Virus Core Protein and Participates in Viral Replication
Author(s) -
Tsuda Yoshimi,
Mori Yoshio,
Abe Takayuki,
Yamashita Tetsuo,
Okamoto Toru,
Ichimura Tohru,
Moriishi Kohji,
Matsuura Yoshiharu
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03789.x
Subject(s) - biology , virology , nucleolus , viral replication , phosphoprotein , cytoplasm , virus , japanese encephalitis , viral structural protein , mutant , microbiology and biotechnology , viral protein , encephalitis , viral entry , gene , genetics , phosphorylation
Japanese encephalitis virus (JEV) core protein is detected not only in the cytoplasm but also in the nucleoli of infected cells. We previously showed that a mutant JEV lacking the nucleolar localization of the core protein impaired viral replication in mammalian cells. In this study, we identified a nucleolar phosphoprotein B23 as a protein binding with the core protein of JEV but not with that of dengue virus. The region binding with JEV core protein was mapped to amino acid residues 38 to 77 of B23. Upon JEV infection, some fraction of B23 was translocated from the nucleoli to the cytoplasm, and cytoplasmic B23 was colocalized with the core protein of wild‐type JEV but not with that of the mutant JEV. Furthermore, overexpression of dominant negatives of B23 reduced JEV replication. These results suggest that B23 plays an important role in the intracellular localization of the core protein and replication of JEV.