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Involvement of Mannose Receptor in Glycopeptidolipid‐Mediated Inhibition of Phagosome‐Lysosome Fusion
Author(s) -
Shimada Kenichi,
Takimoto Hiroaki,
Yano Ikuya,
Kumazawa Yoshio
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03782.x
Subject(s) - mannose , mannose receptor , opsonin , phagosome , lysosome , endosome , biology , microbiology and biotechnology , phagocytosis , receptor , macrophage , mannose 6 phosphate receptor , biochemistry , in vitro , enzyme
We previously reported that glycopeptidolipid (GPL) isolated from Mycobacterium avium serovar 4 inhibited phagosome‐lysosome (P‐L) fusion when macrophages phagocytosed heat‐killed Staphylococcus aureus (SA). In the present study we analyzed the underlying inhibitory mechanism of GPL coated on SA. Elimination of oligosaccharide from GPL abrogated its inhibitory activity. GPL did not inhibit P‐L fusion of opsonized SA phagocytosed via complement receptors. The inhibitory activity of GPL was competitively reduced by the presence of α‐methyl‐ D ‐mannoside and anti‐mannose receptor antibody, suggesting that inhibition of P‐L fusion by GPL is mediated through mannose receptor. Recruitment of early endosome antigen 1 and Ca 2+ /calmodulin kinase II in human macrophage‐like THP‐1 cells were significantly suppressed by GPL, indicating that GPL inhibits steps for leading to the P‐L fusion.