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Organic Solvent‐Selective Domain of the Resistance‐Nodulation‐Division‐Type Xenobiotic‐Antibiotic Transporters of Pseudomonas aeruginosa
Author(s) -
Li Xiaohong,
Eda Shima,
Nakaeu Taiji
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03770.x
Subject(s) - periplasmic space , xenobiotic , transmembrane domain , transporter , biology , pseudomonas aeruginosa , transmembrane protein , biochemistry , membrane transport protein , cytoplasm , membrane , bacteria , escherichia coli , gene , receptor , genetics , enzyme
The hydrophobic substrate‐selective domain of the resistance‐nodulation‐division‐type xenobiotic transporter of Pseudomonas aeruginosa was assigned based on the different organic‐solvent selectivities of MexB and MexY. The MexB‐MexY hybrid protein consisting of two large periplasmic domains of MexB and the transmembrane domains of MexY showed MexB‐type organic solvent selectivity. The results imply that the resistance‐nodulation‐division‐type xenobiotic transporters recognize hydrophobic substrates such as organic solvents by their periplasmic domains and expel them to the external milieu. This is an elegant way to protect the cytoplasmic membrane from membrane‐deteriorating agents.