Premium
Molecular Identification of Ehrlichia Species and ‘Candidatus Neoehrlichia mikurensis’ from Ticks and Wild Rodents in Shizuoka and Nagano Prefectures, Japan
Author(s) -
Naitou Hirotaka,
Kawaguchi Daizoh,
Nishimura Yuusaku,
Inayoshi Megumi,
Kawamori Fumihiko,
Masuzawa Toshiyuki,
Hiroi Midori,
Kurashige Hideaki,
Kawabata Hiroki,
Fujita Hiromi,
Ohashi Norio
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03769.x
Subject(s) - biology , ehrlichia , anaplasmataceae , candidatus , virology , tick , rickettsiales , 16s ribosomal rna , microbiology and biotechnology , bacteria , anaplasma , genetics
A total of 293 ticks and 111 wild rodents that were collected in Shizuoka and Nagano Prefectures, Japan, were examined for infection of Ehrlichia species and ‘Candidatus Neoehrlichia mikurensis.’ The 16S rDNA or the omp‐1 gene of these bacterial DNAs were detected from the spleens of tick‐inoculated mice (5 positive/total 29 mice) or from the spleens of wild rodents (25 positive/total 111 rodents) by PCR amplification. Sequencing of the 16S rDNA revealed Ehrlichia spp. from the 5 tick‐inoculated mice and 8 wild rodents, and ‘Candidatus N. mikurensis’ from 17 wild rodents. The data suggest the presence of additional genetic variants, and potential vectors and/or reservoirs for these bacteria in central Japan.