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Correlation between the Anti‐Virus‐Induced Cytopathic Effect Activity of Interferon‐α Subtypes and Induction of MxA Protein In Vitro
Author(s) -
Schanen Cécile,
Chieux Vincent,
Lobert PierreEmmanuel,
Harvey Jeanne,
Hober Didier
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03766.x
Subject(s) - vesicular stomatitis virus , cytopathic effect , biology , interferon , intracellular , virus , in vitro , virology , biological activity , cell culture , antiviral protein , microbiology and biotechnology , rna , gene , biochemistry , genetics
There are several interferon‐α (IFN‐α) subtypes. Mechanism of disparity in biological effects among members of IFN‐α subtypes remains unexplained. Biological activity of IFN‐α is mediated in part by induction of intracellular antiviral proteins. We studied whether differences in biologic effects of IFN‐α subtypes may rely on their antiviral protein inducing effect. Intracellular induction of MxA protein and anti‐virus‐induced cytopathic effect (CPE) activity of 11 IFN‐α subtypes in human amnion WISH cells have been studied. MxA protein quantitation in cell lysates was performed by immunochemiluminescence assay and anti‐virus‐induced CPE activity was assessed by protection against vesicular stomatitis virus (VSV)‐induced CPE. Range of MxA values was high when cells were treated with 10 and 100 IU/ml of each IFN‐α subtype. Levels of MxA correlated with anti‐VSV‐induced CPE obtained with 10 IU/ml IFN‐α subtype. Together our data show a disparity in MxA‐inducing activity of IFN‐α subtypes and suggest that differences in anti‐VSV‐induced CPE of IFN‐α subtypes in WISH cells can be related to their different ability to induce MxA.