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Rapid and Simple Identification of Orientia tsutsugamushi from Other Group Rickettsiae by Duplex PCR Assay Using groEL Gene
Author(s) -
Park HyoSoon,
Lee JungHee,
Jeong EunJu,
Kim JungEun,
Hong SungJin,
Park TaeKyu,
Kim TaeYung,
Jang WonJong,
Park KyungHee,
Kim BumJoon,
Kook YoonHoh,
Lee SeungHyun
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03760.x
Subject(s) - biology , orientia tsutsugamushi , groel , spotted fever , scrub typhus , virology , rickettsia , duplex (building) , polymerase chain reaction , rickettsiosis , gene , microbiology and biotechnology , genetics , dna , virus , escherichia coli
In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229‐bp and the 366‐bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397‐bp and the 213‐bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.
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