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Effect of Cytokine‐Encoding Plasmid Delivery on Immune Response to Japanese Encephalitis Virus DNA Vaccine in Mice
Author(s) -
Bharati Kaushik,
Appaiahgari Mohan Babu,
Vrati Sudhanshu
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03739.x
Subject(s) - japanese encephalitis , virology , dna vaccination , biology , virus , immune system , antibody titer , antibody , immunization , plasmid , titer , immunology , neutralizing antibody , immunity , granulocyte macrophage colony stimulating factor , cytokine , encephalitis , gene , biochemistry
We have previously shown that immunization of mice with plasmid pMEa synthesizing Japanese encephalitis virus (JEV) envelope protein induced anti‐JEV humoral and cellular immune responses. We now show that intra‐muscular co‐administration of mice with pMEa and pGM‐CSF, encoding murine granulocyte‐macrophage colony‐stimulating factor or pIL‐2, encoding murine interleukin‐2 given 4 days after pMEa, augmented anti‐JEV antibody titers. This did not enhance the level of protection in immunized mice against JEV. However, intra‐dermal co‐administration of pMEa and pGM‐CSF in mice using the gene gun, enhanced anti‐JEV antibody titers resulting in an increased level of protection in mice against lethal JEV challenge.