Premium
Quantitative Microarray‐Based DNA‐DNA Hybridization Assay for Measuring Genetic Distances among Bacterial Species and Its Application to the Identification of Family Enterobacteriaceae
Author(s) -
Amano Makoto,
Ohkusu Kiyofumi,
Kusaba Koji,
Ikeda Hironori,
Nagasawa Zenzo,
Aoki Yosuke,
Kawamura Yoshiaki,
Kobatake Shinzo,
Tanaka Takumi,
Matsuura Shuuji,
Ezaki Takayuki
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03727.x
Subject(s) - biology , enterobacteriaceae , dna–dna hybridization , dna microarray , dna , nucleic acid thermodynamics , chromosome , hybridization probe , circular bacterial chromosome , genetics , gene , microbiology and biotechnology , escherichia coli , gene expression , base sequence
Quantitative DNA‐DNA hybridization to measure the genetic distances among bacterial species is indispensable for taxonomical determination. In the current studies, we developed a method to determine bacterial DNA relatedness on a glass microarray. Reference DNAs representing a total 93 species of Enterobacteriaceae were arrayed on a glass microplate, and signal intensities were measured after 2 hr of hybridization with Cy3‐labeled bacterial DNAs. All immobilized DNAs from members of the family Enterobacteriaceae were identified by this method except for DNAs from Yersinia pseudotuberculosis and Y. pestis . These results suggest that quantitative microarray hybridization could be an alternative to conventional DNA‐DNA hybridization for measuring chromosome relatedness among bacterial species.