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Genotyping of Norovirus Strains Detected in Outbreaks between April 2002 and March 2003 in Osaka City, Japan
Author(s) -
Seto Yoshiyuki,
Iritani Nobuhiro,
Kubo Hideyuki,
Kaida Atsushi,
Murakami Tsukasa,
Haruki Kosuke,
Nishio Osamu,
Ayata Minoru,
Ogura Hisashi
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03718.x
Subject(s) - outbreak , genotype , genotyping , biology , feces , acute gastroenteritis , virology , veterinary medicine , microbiology and biotechnology , genetics , gene , medicine
Noroviruses (NVs) are the major cause of food‐ and waterborne nonbacterial gastroenteritis in Japan. Between April 2002 and March 2003, a total of 111 fecal specimens from 40 outbreaks of acute nonbacterial gastroenteritis in Osaka City, Japan were subject to NV detection. Seventy‐two samples (64.9%) from 31 outbreaks (77.5%) were NV positive by a real time reverse transcription (RT)‐PCR assay. To further determine the genotype of individual NV strains, we sequenced the capsid N‐terminal/shell (N/S) domain of some representative strains from each outbreak. The 51 NV strains detected in this study were segregated into 15 genotypes (6 in genogroup I and 9 in genogroup II), and GII/5 genotype NV was a dominant outbreak genotype.