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Facilitated Production of Secretory IgA against Shiga Toxin B Subunits by Intranasal Application of Antigen‐Coated Polystyrene Microspheres
Author(s) -
Kurohane Kohta,
Kobayashi Chie,
Imai Yasuyuki
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03714.x
Subject(s) - cholera toxin , immunogenicity , antigen , toxin , nasal administration , microsphere , microbiology and biotechnology , immunization , secretory iga , immunology , immune system , immunoglobulin a , biology , antibody , chemistry , immunoglobulin g , chemical engineering , engineering
We examined the effects of microspheres as antigen carriers in mucosal immunization. Shiga toxin B subunits (Stx1B) were adsorbed on 6 μm polystyrene microspheres, which were then intranasally administered to mice together with cholera toxin (CT). Stx1B‐specific serum IgG production and secretory IgA production at local mucosal sites were enhanced by the use of microspheres. When OVA was used as a model antigen, secretory IgA production but not serum IgG production was enhanced on the use of microspheres. These results indicated that microspheres provide a useful means of potentiating the immune response against Stx1B with weak immunogenicity.