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Legionella pneumophila Serogroup 1 Isolates from Cooling Towers in Japan Form a Distinct Genetic Cluster
Author(s) -
AmemuraMaekawa Junko,
Kura Fumiaki,
Chang Bin,
Watanabe Haruo
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03699.x
Subject(s) - pulsed field gel electrophoresis , biology , legionella pneumophila , microbiology and biotechnology , typing , cluster (spacecraft) , legionella , serotype , multilocus sequence typing , gel electrophoresis , molecular epidemiology , bacteria , genotype , genetics , gene , computer science , programming language
Thirty‐one epidemiologically unrelated Legionella pneumophila serogroup 1 isolates (10 from cooling towers, 10 from public spas and/or hot spring baths, and 11 from patients) were analyzed by pulsed‐field gel electrophoresis (PFGE) and sequence‐based typing (SBT) using 6 loci, flaA, pilE, asd, mip, mompS , and proA . The results of PFGE and SBT analysis indicated that all 10 isolates from cooling towers clustered into a unique type, which was distinct from strains of other environmental sources.