Premium
Analysis of Polymorphism of 18S rRNA Gene in Wuchereria bancrofti Microfilariae
Author(s) -
Bhandari Yashodhar,
Dabir Pankaj,
Nandhakumar Krithika,
Dayananda Kannayakanahalli Maheshwarappa,
Shouche Yogesh Shripad,
Reddy Maryada Venkata Rami
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03682.x
Subject(s) - biology , wuchereria bancrofti , restriction fragment length polymorphism , brugia malayi , 18s ribosomal rna , restriction enzyme , polymerase chain reaction , phylogenetic tree , gene , genetics , filariasis , microbiology and biotechnology , helminths , immunology
The polymorphism of the 18S rRNA gene in Wuchereria bancrofti microfilariae (mf) collected from three different zones in India was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The RFLPs of the amplified products obtained after digestion with restriction enzymes Ssp I, Msp I and Hha I showed no difference in the banding patterns among the mf isolates from different endemic zones. Further the sequencing of PCR products did not show any difference in the nucleotide sequence either. The phylogenetic analysis of the sequences of W. bancrofti mf isolates from different endemic zones has shown branching with the earlier reported sequences of W. bancrofti and its close relative Brugia malayi .