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Effects of Type II Alveolar Epithelial Cells on T Cell Mitogenic Responses to Concanavalin A and Purified Protein Derivatives
Author(s) -
Sato Katsumasa,
Shimizu Toshiaki,
Sano Chiaki,
Tomioka Haruaki
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03679.x
Subject(s) - concanavalin a , peripheral blood mononuclear cell , biology , immune system , cell culture , pulmonary alveolus , cell , immunology , cell type , microbiology and biotechnology , t cell , a549 cell , lymphokine , in vitro , macrophage , biochemistry , genetics
To investigate the role of type II alveolar epithelial cells during the T cell‐dependent host immune response to Mycobacterium tuberculosis (MTB), effects of MTB‐infected A‐549 human type II alveolar epithelial cells (A‐549 cells) on T cell mitogenesis in response to concanavalin A (Con A) and purified protein derivatives (PPD) were studied. Human peripheral blood mononuclear cells (PBMCs) were co‐cultivated with uninfected or MTB‐infected A‐549 cells and Con A‐ and PPD‐induced T cell mitogeneses were examined, and the following findings were obtained. T cell mitogenesis was inhibited by uninfected as well as MTB‐infected A‐549 cells, even when a dual‐chamber culture system was used to prevent direct cell contact between PBMCs and A‐549 cells. Therefore, it appears that A‐549 cells suppress T cell mitogenesis by producing some unknown humoral suppressor factors.