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Rapid PrP Sc Detection in Lymphoid Tissue and Application to Scrapie Surveillance of Fallen Stock in Japan: Variable PrP Sc Accumulation in Palatal Tonsil in Natural Scrapie
Author(s) -
Shimada Kimi,
Hayashi Hiroko K.,
Ookubo Yuka,
Iwamaru Yoshifumi,
Imamura Morikazu,
Takata Masuhiro,
Schmerr Mary Jo,
Shinagawa Morikazu,
Yokoyama Takashi
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03660.x
Subject(s) - scrapie , tonsil , biology , virology , microbiology and biotechnology , pathology , immunology , medicine , prion protein , disease
Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP Sc ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinically scrapie‐infected sheep harbored PrP Sc in the brain and palatal tonsil, the two sheep in the pre‐clinical stage harbored PrP Sc in the brain, but not in the palatal tonsil. This study shows that PrP Sc accumulation in palatal tonsil is variable in natural scrapie, even among genetically susceptible sheep.