Premium
Vaccine Efficacy of Recombinant GAPDH of Edwardsiella tarda against Edwardsiellosis
Author(s) -
Liu Ying,
Oshima Syunichirou,
Kurohara Kenrou,
Ohnishi Kouhei,
Kawai Kenji
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03652.x
Subject(s) - edwardsiella tarda , biology , glyceraldehyde 3 phosphate dehydrogenase , escherichia coli , recombinant dna , microbiology and biotechnology , virology , flounder , dehydrogenase , gene , enzyme , genetics , fish <actinopterygii> , biochemistry , fishery
Thirty‐seven kilodalton glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) of Edwardsiella tarda was suggested to be an effective vaccine candidate against E. tarda infection in previous research. For developing a vaccine, obtaining GAPDH in large quantities is necessary. In this study, we determined the complete nucleotide sequence of the gene that encodes GAPDH of E. tarda , and overexpressed the GAPDH of E. tarda by using the Escherichia coli expression system. We immunized Japanese flounder with recombinant GAPDH (rGAPDH) and evaluated its vaccine efficacy. Our results showed that rGAPDH effectively protected Japanese flounder from experimental E. tarda infection, and will contribute to the development of a vaccine against E. tarda .