Premium
Establishment of Highly Specific and Quantitative Immunoassay Systems for Staphylococcal Enterotoxin A, B, and C Using Newly‐Developed Monoclonal Antibodies
Author(s) -
Sasaki Takanori,
Terano Yoshitake,
Shibata Tadayoshi,
Kawamoto Hiroyoshi,
Kuzuguchi Tsuyoshi,
Kohyama Erina,
Watanabe Toshihiro,
Ohyama Tohru,
Gemba Munekazu
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03650.x
Subject(s) - enterotoxin , immunoassay , monoclonal antibody , raw milk , microbiology and biotechnology , biology , antibody , ingestion , food science , immunology , biochemistry , escherichia coli , gene
Staphylococcal enterotoxin (SE) activities remain after boiling or treating with proteases. The main symptoms such as vomiting and diarrhea, are caused by the ingestion of SEs. Among SEs, SEA has been reported to be the major and most toxic protein. A highly specific and simple assay system is required to diagnose staphylococcal food poisoning. Therefore, the development of a suitable assay system is strongly anticipated. In this study, we have established a highly specific and sensitive avidin‐biotin sandwich ELISA (ABS‐ELISA) system for SEA, SEB, and SEC1 using newly‐developed monoclonal antibodies. The linearity of these systems obtained was in the range of 0.78–25 ng/ml for each SE, and furthermore, the lower concentrations of SEs could also be detected. The recoveries of SEs from murine serum, skim milk solution, and raw milk were found to be over 90%, suggesting that our systems could detect SEs without any interventions, such as these from milk or serum proteins. We were also able to quantify SEs in 22 specimens of culture supernatants of S. aureus isolated in past occurrences. Our established system should be very useful not only in the clinical field but also in various fields of investigation because of its quantification and simplicity in detecting SEs.