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A Hemolysin of Vibrio mimicus (VMH) Stimulates Cells to Produce ATP and Cyclic AMP Which Appear to Be Secretory Mediators
Author(s) -
Li Yunshan,
Okamoto Keinosuke,
Takahashi Eizo,
Miyoshi Shinichi,
Shinoda Sumio,
Tsuji Takao,
Fujii Yoshio
Publication year - 2005
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2005.tb03631.x
Subject(s) - glibenclamide , hemolysin , hemolysis , biology , toxin , secretion , cell culture , cyclic nucleotide , stimulation , mechanism of action , vibrionaceae , microbiology and biotechnology , nucleotide , bacteria , endocrinology , medicine , biochemistry , in vitro , virulence , immunology , genetics , gene , diabetes mellitus
The hemolysin of Vibrio mimicus (VMH) is a pore‐forming toxin with both enterotoxic and hemolytic activity. The hemolysis by VMH is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of VMH has remained unclear. In order to clarify the mechanism, we incubated T84 cells (a human colon carcinoma cell line) with VMH and found that the levels of ATP and cyclic AMP of culture medium increased after exposure of the cells to VMH. Subsequently, we found that the fluid accumulating activity of VMH in a mouse internal loop assay was reduced by administration of glibenclamide, an inhibitor of cyclic AMP‐dependent chloride channels, into the intestinal loop. These results suggest that the stimulation of cells to produce nucleotides by VMH is linked to the enterotoxic activity of the toxin.