Premium
Isolation of Mini‐Tn5Km2 Insertion Mutants of Salmonella enterica Serovar Oranienburg Sensitive to NaCl‐Induced Osmotic Stress
Author(s) -
Asakura Hiroshi,
Panutdaporn Nantika,
Kawamoto Keiko,
Igimi Shizunobu,
Yamamoto Shigeki,
Makino Souichi
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03629.x
Subject(s) - salmonella enterica , biology , mutant , microbiology and biotechnology , salmonella , transposable element , strain (injury) , gene , serotype , osmotic shock , isolation (microbiology) , mutation , transposon mutagenesis , bacteria , genetics , anatomy
We previously reported that the viability of Salmonella Oranienburg strains under NaCl stress was variable and depended on the strain's origin; food strains were resistant and patient strains sensitive to NaCl. Therefore, we mutagenized a food strain with a mini‐Tn5Km2 transposon. Of 2,400 mutants screened, 15 NaCl‐sensitive mutants were isolated, and 7 genes associated with NaCl‐sensitivity were identified. The intact genes complemented their own food‐strain mutants, but not patient‐strain mutants, suggesting that the difference in NaCl‐sensitivity between food and patient S . Oranienburg strains might not arise from a single gene mutation, but from change in multiple osmoregulatory mechanisms in Salmonella .