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Comparison of Japanese Isolates of Coxiella burnetii by PCR‐RFLP and Sequence Analysis
Author(s) -
Andoh Masako,
Nagaoka Hiromi,
Yamaguchi Tsuyoshi,
Fukushi Hideto,
Hirai Katsuya
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03627.x
Subject(s) - coxiella burnetii , biology , q fever , nucleic acid sequence , sequence analysis , genetics , virology , sequence (biology) , genetic variation , gene , restriction fragment length polymorphism , microbiology and biotechnology , polymerase chain reaction
The genetic variation of Japanese isolates of Coxiella burnetii , the agent of Q fever, was found for the first time. Forty‐nine out of 72 isolates had the chronic pattern of the isocitrate hydrogenase gene. Sequence analysis revealed that the isolates have a specific nucleotide sequence. The putative amino acid sequence was the same as that of chronic reference strains. These results suggest the variation of C. burnetii isolates in Japan.