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Roles of Toxoplasma gondii ‐Derived Heat Shock Protein 70 in Host Defense against T. gondii Infection
Author(s) -
Ahmed Azza Kamal,
Mun HyeSeong,
Aosai Fumie,
Piao LianXun,
Fang Hao,
Norose Kazumi,
Yano Akihiko
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03611.x
Subject(s) - toxoplasma gondii , biology , hsp70 , spleen , heat shock protein , immune system , microbiology and biotechnology , intraperitoneal injection , toxoplasmosis , immunology , parasite hosting , antibody , pharmacology , biochemistry , world wide web , computer science , gene
C57BL/6 mice receiving intraperitoneal injection of Toxoplasma gondii ‐derived heat shock protein 70 ( T.g . HSP70) on day 3 post T. gondii infection succumbed by day 9 post infection, while vector protein‐injected control mice survived more than 6 months. The deteriorating effect of T.g .HSP70 on host immune responses was dose‐dependent. By T.g . HSP70 injection, T. gondii loads increased in various organs of T. gondii ‐infected mice. Th2 cytokines such as IL‐4 and IL‐10 were continuously produced from spleen and peritoneal exudate cells of T. gondii ‐infected mice by injection of T.g . HSP70. Furthermore, nitric oxide production from peritoneal macrophages in T. gondii ‐infected mice was reduced by T.g . HSP70.