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Autolysis of Porphyromonas gingivalis Is Accompanied by an Increase in Several Periodontal Pathogenic Factors in the Supernatant
Author(s) -
Kamaguchi Arihide,
Nakano Miyo,
Shoji Mikio,
Nakamura Reiko,
Sagane Yoshimasa,
Okamoto Masaaki,
Watanabe Toshihiro,
Ohyama Tohru,
Ohta Michio,
Nakayama Koji
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03550.x
Subject(s) - autolysis (biology) , porphyromonas gingivalis , biology , microbiology and biotechnology , biochemistry , bacteria , enzyme , genetics
Porphyromonas gingivalis autolyzes in the culture media. To examine in more detail the molecular components of the autolysate, two‐dimensional gel electrophoresis was performed. Many protein spots varied both in number and volume. One of these spots included Arg‐gingipain (Rgp) as determined by N‐terminal amino acid sequencing. Corresponding to the increase in spot volume, Rgp activity also increased during autolysis. The results of this study suggested that Rgp and other proteins in the P. gingivalis autolysate may be involved with the prolongation of periodontal disease, even after the death of P. gingivalis cells.