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Detection of the Impairment of CD80 Expression on Circulating Monocytes in HIV‐Infected Thai Children
Author(s) -
Ubol Sukathida,
Aiamkitsumrit Benjamaz,
Nitayaphan Sorachai,
Sirinavin Sayomporn,
Kitthawee Sangvorn
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03543.x
Subject(s) - cd80 , asymptomatic , immunology , flow cytometry , biology , human immunodeficiency virus (hiv) , medicine , cd40 , in vitro , genetics , cytotoxic t cell
The mechanism of progressive anergic response in HIV‐infected children has yet to be adequately described. One possibility is inappropriate delivery of an essential second signal for T‐cell activation due to the inappropriate presentation of co‐stimulatory molecules. To determine whether the ligand for the secondary signal is impaired in pediatric AIDS, we compared the level of CD80 expression by circulating monocytes in HIV‐infected and ‐noninfected children (15 mild/asymptomatic, 13 symptomatic and 12 HIV seronegative children). By two‐color flow cytometry analysis, there was no statistically significant difference in the percentage of monocytes expressing CD80 among the groups (i.e., 63.2±15.8, 60.9±12.7, 61.04±10.9 for uninfected children, mild‐asymptomatic children and symptomatic children, respectively). However, both infected groups showed statistically significant lower levels of CD80 expression, with mean fluorescent intensities of 40.9±15.9 and 38.8±10.7 compared to 57.05±16.3 for the uninfected control group. Our data demonstrated a correlation between HIV infection and impairment of CD80 by circulating monocytes. Whether the impairment on CD80 expression contributes to destruction of the immunological network in HIV‐infected children requires further investigation.