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Identification of Signature Genes for Rapid and Specific Characterization of Yersinia pestis
Author(s) -
Zhou Dongsheng,
Han Yanping,
Dai Erhei,
Pei Decui,
Song Yajun,
Zhai Junhui,
Du Zongmin,
Wang Jin,
Guo Zhaobiao,
Yang Ruifu
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03522.x
Subject(s) - yersinia pestis , biology , polymerase chain reaction , yersinia pseudotuberculosis , gene , genome , genetics , genomic dna , dna microarray , computational biology , dna , virulence , gene expression
Polymerase chain reaction (PCR) amplification of DNA‐based unique markers, the signature sequences, is ideal for rapid detection and identification of pathogens. We described the discovery of twenty‐eight signature genes of Yersinia pestis by DNA microarray‐based comparative genome hybridization in conjunction with PCR validation. Three pairs of Y. pestis ‐specific primers designed from signature genes were demonstrated to have the expected specificity to this target bacterium, without cross‐reaction with the closely related Y. pseudotuberculosis or a large collection of genomic DNAs from other organisms.