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Virulence Properties of Rough and Smooth Strains of Vibrio cholerae O1
Author(s) -
Islam Mohammad Sirajul,
Ahsan Sunjukta,
Khan Sirajul Islam,
Ahmed Qazi Shafi,
Rashid Mohammad Harunur,
Islam Khan M. Nasirul,
Sack Richard Bradley
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03518.x
Subject(s) - enterotoxin , vibrio cholerae , microbiology and biotechnology , biology , virulence , cholera toxin , vibrionaceae , toxin , vibrio , titer , pathogenicity , bacteria , gene , escherichia coli , antibody , genetics
A comparative study was carried out to see the differences in pathogenicity of rough and smooth strains. A total of 10 strains including 5 each of rough and smooth strains of Vibrio cholerae O1 were tested and found positive for toxin production by enzyme‐linked immunosorbent assay (ELISA) in Richardson's and AKI media. All the smooth and rough strains, except one, showed a titre of 1:10 and 1:100 in Richardson's and AKI media, respectively. Both types of strains produced enterotoxin in rabbit ileal loop (RIL). The differences in multiplication abilities of smooth and rough strains in RIL were statistically significant ( P <0.05). However, these differences in multiplying abilities did not influence the adherence potential or enterotoxin production as there was no significant difference ( P >0.05) between these properties. This study demonstrated that the rough strains are equally pathogenic and as important as smooth strains.